Domesticate and develop probiotics to reduces greenhouse gas emissions and improves livestock health.

Title: Directed Evolution of Methylococcus capsulatus as a Probiotic for Reducing Methane Emissions in Livestock Introduction Methane emissions from livestock, particularly from enteric fermentation in cattle, contribute significantly to global greenhouse gas emissions. Implementing strategies to reduce methane production in livestock has the potential to mitigate climate change while maintaining agricultural productivity. This proposal aims to use directed evolution to engineer Methylococcus capsulatus, a methanotrophic bacterium, to act as a probiotic in cattle and livestock that can reduce methane emissions. Objective To develop a novel Methylococcus capsulatus strain with enhanced methane consumption capabilities and the ability to survive and thrive in the gastrointestinal tract of cattle and livestock. This will be achieved by: Engineering Methylococcus capsulatus to optimize methane oxidation. Enhancing the bacterium's resistance to the gastrointestinal environment in cattle and livestock. Evaluating the effectiveness of the engineered Methylococcus capsulatus as a probiotic in reducing methane emissions. Methods Directed Evolution of Methylococcus capsulatus

Expected Outcomes and Impact The successful development of an engineered Methylococcus capsulatus strain with improved methane oxidation capabilities and resistance to gastrointestinal conditions has the potential to significantly reduce methane emissions from cattle and livestock. This would contribute to climate change mitigation efforts and promote sustainable livestock farming practices. Moreover, the use of Methylococcus capsulatus as a probiotic may have additional benefits, such as improving feed efficiency and animal health, which would enhance the overall sustainability and profitability of the livestock industry. In conclusion, this proposal outlines a novel approach to reducing methane emissions from livestock using directed evolution to engineer Methylococcus capsulatus as a probiotic. If successful, this strategy could make a significant impact on global greenhouse gas emissions and support sustainable agriculture.

At Defi Biolabs, our mission is to revolutionize the livestock industry by harnessing the power of next generation probiotics to reduce greenhouse gas emissions and improve the wellbeing of livestock. We are dedicated to creating innovative, sustainable, and eco-friendly solutions that benefit both the environment and the global agricultural community. Through cutting-edge research and techbio we strive to make a lasting, positive impact on the world by fostering responsible and sustainable livestock management practices for a greener, healthier earth.